diff --git a/packages/vite/src/node/plugins/css.ts b/packages/vite/src/node/plugins/css.ts
index 85ba5eea8e5b89..d9ebbb83220390 100644
--- a/packages/vite/src/node/plugins/css.ts
+++ b/packages/vite/src/node/plugins/css.ts
@@ -739,10 +739,13 @@ export function cssPostPlugin(config: ResolvedConfig): Plugin {
           .map((file) => path.basename(file))
           .join('|')
           .replace(/\./g, '\\.')
+        // require and import calls might be chained by minifier using the comma operator
+        // in this case we have to keep one comma
+        // if a next require is chained or add a semicolon to terminate the chain.
         const emptyChunkRE = new RegExp(
           opts.format === 'es'
-            ? `\\bimport\\s*["'][^"']*(?:${emptyChunkFiles})["'];\n?`
-            : `\\brequire\\(\\s*["'][^"']*(?:${emptyChunkFiles})["']\\);\n?`,
+            ? `\\bimport\\s*["'][^"']*(?:${emptyChunkFiles})["'];`
+            : `(\\b|,\\s*)require\\(\\s*["'][^"']*(?:${emptyChunkFiles})["']\\)(;|,)`,
           'g',
         )
         for (const file in bundle) {
@@ -769,7 +772,10 @@ export function cssPostPlugin(config: ResolvedConfig): Plugin {
             chunk.code = chunk.code.replace(
               emptyChunkRE,
               // remove css import while preserving source map location
-              (m) => `/* empty css ${''.padEnd(m.length - 15)}*/`,
+              (m) =>
+                opts.format === 'es'
+                  ? `/* empty css ${''.padEnd(m.length - 15)}*/`
+                  : `${m.at(-1)}/* empty css ${''.padEnd(m.length - 16)}*/`,
             )
           }
         }