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MMseqs2 Clustering - Complete Command List (WSL) 1. Remove alignment gaps from the input FASTA sed s/-//g /mnt/c/Users/file/Documents/aligned_codons.fasta /mnt/c/Users/file/Documents/no_gaps_sequences.fasta ...
  • Gumindu99
  • 2
  • Opened 
    yesterday
  • #977

Hi there, I am embarking on putting together a taxonomic database using MAGs with GTDB-style taxonomy annotations. Specifically, I want to combine my MAGs with the current version of GTDB. To do that I ...
  • clb21565
  • Opened 
    2 days ago
  • #976

Hello, I m using the following command to find the closest references for multiple reads. As both the database and the fasta file are quite big, it takes quite some time. I tried using max-seqs=7, however, ...
  • LauraVP1994
  • Opened 
    3 days ago
  • #975

Hi ! I want to use MMseqs2 to obtain protein sequences from the query dataset that are completely dissimilar to the protein sequences in the target dataset (e.g., with a similarity threshold of 0.3). ...
  • liyue9129
  • 3
  • Opened 
    7 days ago
  • #973

Hi, I started to use mmseqs2 to functionally annotate genes, and saw a surprising requirement of disk space. I tested the swissprot db with a concatenated fna file using nohup mmseqs easy-search /mnt/8T_2/zuo/gene_cluster_cohort/27_genes_cohort.fna ...
  • hellopeccat
  • 2
  • Opened 
    12 days ago
  • #972

As instructed by the user manual and this blogpost, I used the following script to compute pairwise sequence identity for a dataset (called bindingnet here): fake_pref() { QDB= $1 TDB= $2 RES= $3 ...
  • wehs7661
  • 4
  • Opened 
    15 days ago
  • #971

As far as I know, MMseq2 can be used instead of Blastn , and it has faster speed than Blastn . (even, two tools have different usage) For understanding the algorithm of MMseq2, I was reading your paper, ...
  • JKL-uxf
  • 1
  • Opened 
    15 days ago
  • #970

Hi there, I have first downloaded nr.gz using mmseqs databases NR nr tmp, without any error found. However, when mmseqs2 went on to automatically performed createdb tmp/18325772313164835411/nr.gz nr --compressed ...
  • hellopeccat
  • 6
  • Opened 
    16 days ago
  • #969

Hi, I wonder if it s possible to add a deduplication step before calculating MSAs for colabfold. I noticed that when generating MSAs for a large batch of alphafold2-multimer-v3 analyses, there are quite ...
  • yanj14jy15
  • Opened 
    22 days ago
  • #968

Hi, thank you for the big work behind MMseqs2. I tried to setup GTDB db (r220) as written in the WiKi. In the folder I successfully get all the files indicated by the workflow: taxonomy/ ├── ar53_taxonomy_r220_reps.tsv ...
  • luigallucci
  • 4
  • Opened 
    24 days ago
  • #967
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